Technical Notes

Acetonitrile Extraction Of Peptides
Compares mass spectrometry results for in-gel digestion with and without acetonitrile extraction of peptides from the gel plug.

Sensitivity for Protein Identification
This data demonstrates our ability to successfully identify proteins from as little as 2ng of starting material loaded onto an SDS-PAGE gel.

2D electrophoresis parameters, gel staining, detection, and differential analysis protocols (PDF)
This report presents the results of a variability study performed at NextGen Sciences It contains detailed methods for large format 2D electrophoresis and software-assisted differential analysis.